A DNA sensor using gold-coated barcode silica nanotubes
نویسندگان
چکیده
The bio barcode silica nanotubes (SNTs) was developed to detect pathogenic bacteria using quantum dots and beacon-type capture probe. Various quantum dots were embedded in SNT (QD-SNT) to generate a barcoding signal with different colors and orders. The QD-SNT was coated by colloidal gold nanoparticle to immobilize hairpin structured capture probe on outer walls. Pathogenic specific capture probes, which have fluorescent dye, were designed and immobilized on the outer wall of each QD-SNTs, respectively. As a result, these barcoded QD-SNTs could detect the E.coli specific nucleic acid products amplified by NASBA (Nucleic Acid Sequence Based Amplification).
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